253. A new PCR-based method for the early detection and identification of spoilage yeast in wine, beer, and alcohol-free beverages

Vincent Ulve (1), Leon Fyfe (2); (1) Pall GeneDisc Technologies, Bruz, France; (2) Pall Corporation, U.S.A.

Yeast, Fermentation, and Microbiology
Supplier Poster

Yeast detection and identification is a major concern for beverage industries aiming for high-quality product delivery and brand protection. The beverage industry needs a reliable method enabling rapid decision-making as opposed to time-consuming conventional microbiological methods. Implementation of the GeneDisc system, utilizing real-time polymerase chain reaction (PCR) technology improves both profitability and quality control management. The GeneDisc technologies offer a highly flexible method to monitor yeast spoilage in the beverage industry. The sample preparation is supported by a universal and fast enrichment step of 28 hr, with two optional filtration steps, prior to and/or after enrichment, enabling a significantly increased level of sensitivity. A longer enrichment time can be implemented for slow-growing microorganism detection. PCR analysis run with the GeneDisc cycler is fast and extremely easy to use, with an integrated inhibition control and automated data analysis to obtain confident results. PCR detection is supported by a GeneDisc plate allowing detection of yeast. For specific yeast identification, a second GeneDisc plate is available, which is designed to detect the 12 major spoiler yeast species and/or genus associated simultaneously. This GeneDisc plate can be used as a specific selective tool for spoilage yeast identification in samples containing processing yeast and as a very efficient tool for direct colony identification. The GeneDisc method for yeast allows a next-day result for the major spoilage yeast. Time to result, sensitivity and specificity are validated on challenging samples with the main yeast spoilers present in the beverage industry.

Vincent Ulvé is a senior principal R&D scientist for Pall GeneDisc Technologies, with five years of experience in biotechnology and PCR development both in France and the United States. He has a Ph.D. degree from the University of Rennes 1 and also has experience in research in the food industry in the French National Institute for Agricultural Research and International Dairy company, with work published in several scientific journals and posters and oral communications in international congress.